Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC3s)

摘要: We developed different types of glass cell-culture chips (GC3s) for culturing cells microscopic observation in open media-containing troughs or microfluidic structures. Platinum sensor and manipulation structures were used to monitor physiological parameters allocate permeabilize cells. Electro-thermal micro pumps distributed chemical compounds the systems. The integrated temperature sensors showed a linear, Pt1000-like behavior. Cell adhesion proliferation monitored using interdigitated electrode (IDESs). cell-doubling times primary murine embryonic neuronal (PNCs) determined based on IDES capacitance-peak shifts. electrical activity PNC networks was detected multi-electrode arrays (MEAs). During seeding, dielectrophoretically allocated individual MEAs improve network MEA pads with diameters 15, 20, 25, 35 µm tested. After 3 weeks, magnitudes action potentials highest 25 diameter did not differ when inter-pad distances 100 170 µm. Using 25-µm circular oxygen electrodes, signal currents media found range from approximately −0.08 nA (0% O2) −2.35 (21% O2). It observed that 60-nm thick silicon nitride-sensor layers stable potentiometric pH under conditions periods days. Their sensitivity between 5 9 as high 45 mV per step. concluded sensorized GC3s are potential animal replacement systems purposes such toxicity pre-screening. For example, effect mefloquine, medication treat malaria, this study GC3 system.