Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers.

摘要: Finger millet (Eleusine coracana), an allotetraploid cereal, is widely cultivated in the arid and semiarid regions of world. Three DNA marker techniques, restriction fragment length polymorphism (RFLP), randomly amplified polymorphic (RAPD), inter simple sequence repeat amplification (ISSR), were employed to analyze 22 accessions belonging 5 species Eleusine. An 8 probe--3 enzyme RFLP combination, 18 RAPD primers, 6 ISSR respectively, revealed 14, 10, 26% 17 E. coracana from Africa Asia. These results indicated a very low level variability finger millets but did allow each line be distinguished. The different Eleusine could easily identified by technology 16% intraspecific exhibited two analyzed floccifolia suggested much higher diversity this than coracana. Between species, indica shared most markers, while tristachya considerable number indicating that these three form close genetic assemblage within compressa found divergent among examined. Comparison RFLP, RAPD, technologies, terms quantity quality data output, ISSRs are particularly promising for analysis plant genome diversity.