Feline leptin: immunogenic and biological activities of the recombinant protein, and its measurement by ELISA.
作者： Haruki SHIBATA , Noriyasu SASAKI , Tsutomu HONJOH , Iwao OHISHI , Mitsuyoshi TAKIGUCHI
DOI: 10.1292/JVMS.65.1207
关键词: Chinese hamster ovary cell 、 Leptin 、 Antibody 、 Western blot 、 Adipose tissue 、 Recombinant DNA 、 Biology 、 Internal medicine 、 Leptin receptor 、 Endocrinology 、 CATS
摘要: Leptin is a protein synthesized and secreted primarily by adipose tissue. The blood leptin concentration known to reflect body fat content in rodents, humans dogs, thereby useful for quantitative assessment of obesity. In the present study, we produced recombinant feline Escherichia coli transfected with cDNA cloned previously. molecular weight 16 kDa induced phosphorylation signal transducers activators transcription 3 (STAT3) cells expressing rat receptor. anti-feline antibody raised rabbits reacted well human less rodents' Western blot analysis. Sandwich enzyme-linked immunosorbent assay (ELISA) was developed, using rabbit as standard. this ELISA system, cross-reactivity human, mouse 30.7%, 69.5% 66.6%, respectively. plasma levels 24 healthy cats were range from 0.3 29.7 ng/ml mean +/- SEM 4.5 1.3 ng/ml, being positively proportional content. These results indicate that our system may be obesity cats.
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