Localization of a nuclear protein during development in the pea seedling

摘要: Abstract The subcellular localization of a nuclear antigenic protein has been accomplished through the application immunogold methodology for immunocytochemistry. Protein extracted from internode tissue pea seedlings was used production monoclonal antibody that applied to sections electron microscopy. As second antibody, goat anti-mouse IgG conjugated with colloidal gold served as label. 30 kDa localized in only chromatin differentiating and mature companion cells parenchyma phloem. By contrast, virtually all meristematic, undifferentiated, apical dome exhibited labeling. In young, immediately behind apex labeling found cell types except pith parenchyma. changing this progression differentiation suggests possibility may, some way, be related regulatory events development.