In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating.

摘要: This work aimed to evaluate the in vitro response of Transfected Human Foetal Osteoblast (hFOB) cultured on a magnesium-loaded mesoporous TiO2 coating. The application films titanium implant surfaces has shown very promising potential enhance osseointegration. type coating ability act as framework sustain bioactive agents and different drugs. Magnesium is element that, after calcium, most frequently used dope surfaces, since it crucial for protein formation, growth factor expression, aids bone mineral deposition surfaces. Mesoporous with an average pore-size 6 nm were produced by evaporation-induced self-assembly method (EISA) deposited onto discs. loading was performed soaking discs magnesium chloride solution. Surface characterization conducted SEM, XPS, optical interferometry, AFM. release profile assessed at time points using Detection kit. Cell morphology spreading observed SEM. cytoskeletal organization stained TRITC-conjugated Phalloidin cell viability evaluated through mitochondrial colorimetric (MTT) assay. Furthermore, gene expression markers mineralization analyzed real RT-PCR alizarin-red staining, respectively. surface chemical analysis XPS revealed successful adsorption AFM measurements presence nanostructured roughness. Osteoblasts adhesion well unaffected addition possibly due its rapid burst release, however, enhanced 3D nanostructure layer.