Cellular Response of Human Bone Marrow Derived Mesenchymal Stem Cells to Titanium Surfaces Implanted with Calcium and Magnesium Ions
作者： Sun Won , Yoon-Hyuk Huh , Lee-Ra Cho , Hee-Su Lee , Eung-Sun Byon
DOI: 10.1007/S13770-017-0028-3
关键词:
摘要: Surface characteristics and cellular response to titanium surfaces that had been implanted with calcium magnesium ions using plasma immersion ion implantation deposition (PIIID) were evaluated. Three different analyzed: a resorbable blast media (RBM) surface (blasted hydroxyapatite grit), ion-implanted surface, surface. The investigated by scanning electron microscopy (SEM), roughness testing, X-ray diffraction (XRD), Auger spectroscopy (AES). Human bone marrow derived mesenchymal stem cells cultured on the 3 surfaces. Initial cell attachment was evaluated SEM, proliferation determined MTT assay. Real-time polymerase chain reaction (PCR) used quantify osteoblastic gene expression (i.e., genes encoding RUNX2, type I collagen, alkaline phosphatase, osteocalcin). analysis did not reveal any changes in topography after implantation. AES revealed present deeper layers than ions. ion- showed greater initial attachment. Investigation of no significant difference among groups. After 6 days cultivation, RUNX2 higher osteocalcin lower In conclusion, PIIID technique changed chemistry without changing topography. Calcium
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