4-Trimethylammonium antipyrine: a quaternary ammonium nonradionuclide marker for blood-brain barrier integrity during in vivo microdialysis.

摘要: Abstract The well-controlled microdialysis (MD) study of substance permeation into brain extracellular fluid (ECF) and cerebrospinal requires consideration blood-brain barrier (BBB) integrity, which might be compromised by probe implantation. Others have assessed BBB integrity with radionuclide markers. A nonradionuclide marker may desirable in many studies. charged antipyrine analogue useful to determine concomitant characterization efficiency (Yokel et al., 1992, J Pharmacol Toxicol Methods 27: 135–142), not require another analytical technique. We synthesized, validated, evaluated 4-trimethylammonium (4TMA-AP) as a marker. was determined calculation percentage (P i ) from brain/blood concentrations. P s Evan's blue, does permeate the intact BBB, 4TMA-AP were significantly different rats without known disruption, suggesting lack through BBB. When MD probes slowly implanted frontal cortex, usually zero. Intracarotid oleic acid injection open increased s, that entered ECF when compromised. Rapid implantation produced disruption. predicted appearance suggests it is