The effect of epidermal growth factor receptor (EGFR) expression on in vivo growth of rat C6 glioma cells.

摘要: The discovery of EGFR gene amplification in glioblastoma multiforme has prompted interest experimental therapies to target the receptor on brain tumor cells. To develop an animal model for vivo study such strategies, we transfected C6 glioma cells with a plasmid containing neomycin resistance and human under control glucocorticoid-inducible MMTV promoter. Following selection G418, individual clones that expressed at high levels were selected. Kinetics EGF binding fit dual site indicating presence both (KA = 2.5 x 10(9) M-1) low 3.3 10(7) affinity receptors. assess growth vivo, graded numbers either wild-type or implanted into brains CD Fischer 344 rats. No differences survival observed between groups animals injected inocula 10(3) 10(4) respectively. In addition, one-third (7/21) challenged 10(5) 10(6) survived > 50 days compared 0% (0/12) Such effect suggests greater immunogenicity cells, but only larger inocula. Since will grow outbred inbred strains, our should have number applications including targeting therapy.