Enhanced Fluorescence Imaging of Live Cells by Effective Cytosolic Delivery of Probes

摘要: Background Microscopic techniques enable real-space imaging of complex biological events and processes. They have become an essential tool to confirm complement hypotheses made by biomedical scientists also allow the re-examination existing models, hence influencing future investigations. Particularly live cells is crucial for improved understanding dynamic processes, however hitherto cell has been limited necessity introduce probes within a without altering its physiological structural integrity. We demonstrate herein that this hurdle can be overcome effective cytosolic delivery. Principal Findings We show delivery several types mammalian using nanometre-sized biomimetic polymer vesicles (a.k.a. polymersomes) offer both highly efficient cellular uptake endolysomal escape capability any effect on metabolic activity. Such biocompatible polymersomes encapsulate various including membrane nucleic acid as well labelled acids, antibodies quantum dots. Significance We sufficient quantities cytosol, allowing sustained functional over time periods days weeks. Finally combination such staining with three-dimensional confocal laser scanning microscopy allows in environments under mono-culture co-culture conditions. Thus migration proliferation studied models are much closer vivo situation.