Investigating a suitable model for the study of vitamin D mediated regulation of human placental gene expression
作者： Claire L. Simner , Brogan Ashley , Cyrus Cooper , Nicholas C. Harvey , Rohan M. Lewis
DOI: 10.1016/J.JSBMB.2019.105576
关键词: Cell biology 、 Cell culture 、 HEK 293 cells 、 Gene expression 、 Epithelium 、 Biology 、 Fetus 、 Placenta 、 Embryonic stem cell 、 Syncytiotrophoblast
摘要: Abstract Transfer and metabolism of vitamin D across the human placenta is required for fetal development. However, these fundamental mechanisms are not well understood model systems to help understand them. The BeWo choriocarcinoma cell line derived from extravillous trophoblast but used as a villous syncytiotrophoblast placental barrier. Questions have been raised about suitability trophoblast. This study compares expression amino acid transporters related genes in term with embryonic kidney (HEK)293 lines. HEK293 cells, transporting epithelium may be more similar placenta. Gene was much than BeWo. provides further evidence that an appropriate closely represents now investigate effects on ex-vivo.
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