A protocol to analyse cellular dynamics during plant development
作者： Pierre Barbier de Reuille , Isabelle Bohn-Courseau , Christophe Godin , Jan Traas
DOI: 10.1111/J.1365-313X.2005.02576.X
关键词: Microscope 、 Confocal microscopy 、 Biology 、 Botany 、 Software 、 Preprocessor 、 Segmentation 、 Identification (information) 、 Object (computer science) 、 Protocol (object-oriented programming) 、 Biological system 、 Plant science 、 Genetics 、 Cell biology
摘要: In vivo microscopy generates images that contain complex information on the dynamic behaviour of three-dimensional (3D) objects. As a result, adapted mathematical and computational tools are required to help in their interpretation. Ideally, complete software chain study dynamics 3D object should include: (i) acquisition, (ii) preprocessing (iii) segmentation images, followed by (iv) reconstruction time space (v) final quantitative analysis. Here, we have developed such protocol cell at shoot apical meristem Arabidopsis. The uses serial optical sections made with confocal microscope. It includes specially designed algorithms automate identification lineage analyse surface.
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sci-hub.se 参考文章(11)
Jan Traas, John H Doonan, Cellular basis of shoot apical meristem development. International Review of Cytology-a Survey of Cell Biology. ,vol. 208, pp. 161- 206 ,(2001) , 10.1016/S0074-7696(01)08004-4
Jim Haseloff, Old botanical techniques for new microscopes. BioTechniques. ,vol. 34, pp. 1174- 1182 ,(2003) , 10.2144/03346BI01
Olivier Hamant, Fabien Nogué, Enric Belles-Boix, Delphine Jublot, Olivier Grandjean, Jan Traas, Véronique Pautot, The KNAT2 Homeodomain Protein Interacts with Ethylene and Cytokinin Signaling Plant Physiology. ,vol. 130, pp. 657- 665 ,(2002) , 10.1104/PP.004564
Jacques Dumais, Dorota Kwiatkowska, Analysis of surface growth in shoot apices Plant Journal. ,vol. 31, pp. 229- 241 ,(2002) , 10.1046/J.1365-313X.2001.01350.X
Jean Serra, Luc Vincent, An overview of morphological filtering Circuits Systems and Signal Processing. ,vol. 11, pp. 47- 108 ,(1992) , 10.1007/BF01189221
Claudia van den Berg, Viola Willemsen, Willem Hage, Peter Weisbeek, Ben Scheres, Cell fate in the Arabidopsis root meristem determined by directional signalling. Nature. ,vol. 378, pp. 62- 65 ,(1995) , 10.1038/378062A0
Olivier Grandjean, Teva Vernoux, Patrick Laufs, Katia Belcram, Yuki Mizukami, Jan Traas, In Vivo Analysis of Cell Division, Cell Growth, and Differentiation at the Shoot Apical Meristem in Arabidopsis The Plant Cell. ,vol. 16, pp. 74- 87 ,(2004) , 10.1105/TPC.017962
L. Vincent, P. Soille, Watersheds in digital spaces: an efficient algorithm based on immersion simulations IEEE Transactions on Pattern Analysis and Machine Intelligence. ,vol. 13, pp. 583- 598 ,(1991) , 10.1109/34.87344
G Venugopala Reddy, Marcus G Heisler, David W Ehrhardt, Elliot M Meyerowitz, Real-time lineage analysis reveals oriented cell divisions associated with morphogenesis at the shoot apex of Arabidopsis thaliana. Development. ,vol. 131, pp. 4225- 4237 ,(2004) , 10.1242/DEV.01261
Robert H Webb, Confocal optical microscopy Reports on Progress in Physics. ,vol. 59, pp. 427- 471 ,(1996) , 10.1088/0034-4885/59/3/003