Cell-type specific and combinatorial usage of diverse transcription factors revealed by genome-wide binding studies in multiple human cells

摘要: Cell-type diversity is governed in part by differential gene expression programs mediated transcription factor (TF) binding. However, there are few systematic studies of the genomic binding different types TFs across a wide range human cell types, especially relation to expression. In ENCODE Project, we have identified locations 11 CTCF, RNA Pol II (RNAPII), and MYC, three with diverse roles. Our data analysis revealed how these factors bind features shape cell-type specificity. CTCF bound predominantly intergenic regions while RNAPII MYC preferentially core promoter regions. sites were relatively invariant showed greatest co-localized at many their putative target genes. specific sites, particular for RNAPII, associated functions. Patterns generally conserved types. occupancy was higher over exons than adjacent introns, likely reflecting link between transcriptional elongation splicing. TF positively correlated levels genes, but combinatorial binding, even more strongly These illuminate biology.