CTCF negatively regulates HOXA10 expression in breast cancer cells
作者： Muhammad Mustafa , Ji-Yeon Lee , Myoung Hee Kim
DOI: 10.1016/J.BBRC.2015.10.058
关键词:
摘要: HOX genes not only play important roles in defining body patterning during embryonic development, but also control numerous cellular events adult cells. Deregulated gene expression different cancers including breast cancer is now increasingly being reported. Given that human HOXA cluster marked with several CTCF binding sites, we investigated whether the presence of associated directly Several genes, such as HOXA4, HOXA5 and HOXA10, were deregulated by overexpression knockdown MCF-7 cells. Among these HOXA10 an emerging tumor suppressor for its role activation p53 countering tumorigenesis cancer. Here provided evidences functions a negative regulator The putative promoter region lies between 5.3 6.1 kb upstream start codon activity was negatively regulated CTCF. Together in-silico analysis in vitro mutation assay identified 20 bp motif flanking core element HOXA10. kept inactivated maintaining H3K27me3 inactivation marks Epigenetic silencing cells may contribute towards decreasing apoptosis promoting metastasis.
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