Antibodies Against the C-Terminus of ApoA-1 Are Inversely Associated with Cholesterol Efflux Capacity and HDL Metabolism in Subjects with and without Type 2 Diabetes Mellitus

摘要: Background: We determined relationships of cholesterol efflux capacity (CEC), plasma esterification (EST) and cholesteryl ester transfer (CET) with anti-c-terminus apoA-1 (Ac-terAA1) anti-apolipoprotein (apo)-1 (AAA1) autoantibodies in subjects without Type 2 diabetes mellitus (T2D). Methods: In 75 T2D nondiabetic subjects, Ac-terAA1 AAA1 levels were measured by enzyme-linked immunosorbent assay. CEC was as [3H]-cholesterol from human cultured fibroblasts to diluted individual subject plasma. Plasma EST CET assayed isotope methods. Results: similar between control subjects. Univariate regression analysis (n = 150) demonstrated that inversely correlated CEC, EST, CET, total cholesterol, non-HDL triglycerides apolipoprotein B, (p < 0.05 p 0.01), but not glucose HbA1c. separate multivariable linear models, associated independently age, sex, drug use (β −0.186, 0.026; β −0.261, 0.001; −0.321, respectively). These associations lost after additional adjustment for triglycerides. No observed AAA1. Conclusions: are autoantibodies, conceivably attributable an inverse relationship these B-containing lipoproteins.