Rapid detection of Salmonella in pet food: design and evaluation of integrated methods based on real-time PCR detection.

摘要: Reducing the risk of Salmonella contamination in pet food is critical for both companion animals and humans, its importance reflected by substantial increase demand pathogen testing. Accurate rapid detection foodborne pathogens improves safety, protects public health, benefits producers assuring product quality while facilitating release a timely manner. Traditional culture-based methods screening are laborious can take 5 to 7 days obtain definitive results. In this study, we developed two low levels using real-time PCR: (i) 25 g dried less than 14 h with an automated magnetic bead–based nucleic acid extraction method (ii) 375 composite dry matrix 24 manual centrifugation-based preparation method. Both included preclarification step a...