In Vivo Perturbation of Human Marrow Cell Cycle Progression by Ifosfamide

摘要: The in vivo cytokinetic effects on bone marrow cells from 19 patients with leukemia and lymphoma were investigated following a 5-day continuous i.v. infusion of ifosfamide (a congener cyclophosphamide) at daily dose 1.0 to 1.8 g/sq m. There 8 without involvement by tumor the time study, 11 had various degrees neoplastic replacement. Ifosfamide induced shift DNA compartment distribution as determined pulse cytophotometry, promoting significant increase proportion (G2 + M) phase decrement G1/0 fraction. mitotic index prior after administration seldom exceeded corresponding size, relative changes histogram-derived fractions indices consistent G2 13 16 evaluable observations. Among variables tested (ifosfamide dose, degree replacement cells, size pretreatment S-phase compartment, percentage myeloid erythroid precursors), nucleated red therapy was most closely related ifosfamide-induced accumulation. This suggests that kinetic primarily involve precursor compartment. Absence drug-induced anemia indicates transient delay rather than an irreversible block preceding cell death and/or preferential kill cells. In subgroup more 75% their marrow, increment predominant effect, which not associated clinical response.