Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-α and interleukin-1β are associated with preeclampsia in Serbian women.

摘要: PROBLEM Preeclampsia has a multifactorial origin with genetic, immunological, and environmental factors described as main contributors to its onset. This study aimed investigate glutathione-S-transferase M1 (GSTM1) T1 (GSTT1) gene polymorphisms, the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), potential relationship between GST polymorphisms cytokine levels in preeclampsia uncomplicated pregnancy. METHOD OF STUDY prospective case-control included 50 women healthy pregnant women. DNA RNA were extracted from leukocytes. Deletion analyzed by PCR, while mRNA was real-time PCR. RESULTS GSTM1 null genotype present GSTT1 increased risk for development. without deletion early preeclampsia. Relative TNF-α significantly higher compared (P = 0.006). Expression IL-1β severe late control group (P = 0.005, P = 0.007, respectively). A significant positive correlation observed (Spearman's ρ = 0.312, P = 0.028) IL-6, ρ = 0.296, P = 0.037). patients (P = 0.015) IL-6 (P = 0.015). CONCLUSIONS represents factor development, favors is also associated cytokines, predominantly IL-1β.