Lack of caspase-dependent apoptosis in spinal motor neurons of the wobbler mouse.
作者： Paolo Bigini , Cristiana Atzori , Elena Fumagalli , Alfredo Cagnotto , Sara Barbera
DOI: 10.1016/J.NEULET.2007.08.057
关键词: Central nervous system disease 、 Motor neuron 、 Caspase 、 Cholinergic neuron 、 Neuroscience 、 Pathology 、 Immunostaining 、 Biology 、 Spinal cord 、 Amyotrophic lateral sclerosis 、 Programmed cell death
摘要: The mechanism of motor neuron degeneration in amyotrophic lateral sclerosis (ALS) is still unclear and the post-mortem analysis samples from ALS patients does not permit a clarification early events cell death occurring ALS. Animal models represent reliable tool to investigate type death. Attention was focused on possible role apoptosis spontaneous model cervical spinal cord degeneration, wobbler mouse. Firstly, rate loss region mice during different phases symptoms progression quantified by CholineAcetyltransferase (ChAT) immunohistochemistry. This followed series immunohistological studies ascertain whether actually involved. ChAT immunostaining confirmed severe cholinergic neurons. Since maximal first phase disease (from 3rd 5th postnatal week), apoptotic markers were evaluated 4-week-old mice. study, carried out examining large number sections 20 affected animals healthy littermates, did show either caspase activation or DNA fragmentation. These results strongly suggest that mouse related caspase-dependent mechanism.
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