Interaction of Vibrio spp. with the Inner Surface of the Digestive Tract of Penaeus monodon

摘要: Several species of Vibrio are the causative agent gastroenteritis in humans. In aquaculture, harveyi (Vh) and V. parahaemolyticus (Vp) have long been considered as shrimp pathogens freshwater, brackish marine environments. Here we show by using scanning electron microscopy (SEM) that Penaeus monodon orally inoculated with each these two via an Artemia diet had numerous bacteria attached randomly across stomach surface, single large biofilm-like clusters 6 h post-infection. A subsequent marked proliferation number within formations resulted development infections stomach, upper middle midgut, but neither posterior midgut nor hindgut. SEM also revealed induced production peritrichous pili-like structures Vp attaching to lining, whilst only a polar fibre was seen forming apparent physical bridge between Vh host’s epithelium. contrast observations, no such adherences or linkages were when trials conducted non-pathogenic spp. Micrococcus luteus, obvious resultant changes gut surface. naive shrimp, hindgut found be favorable site for notably curved, short-rod shaped which probably belong Data from current study suggests P. must able colonize digestive tract, particularly where chitin is present, then they use array virulent factors enzymes infect their host resulting disease. Oral infection better way mimicking natural routes infection; investigating host-bacteria interactions occurring tract may lead new strategies prevention control bacterial penaeids.