Chitin utilization by marine bacteria. Chemotaxis to chitin oligosaccharides by Vibrio furnissii.

摘要: Abstract The adhesion/deadhesion apparatus of the marine bacterium Vibrio furnissii (Yu, C., Lee, A., Bassler, B. L., and Roseman, S. (1991) J. Biol. Chem. 266, 24260-24267) probably catalyzes first step in colonizing chitin. Evidence is presented here for a second step, chemotaxis to chitin hydrolysis products. V. swarms toward oligomers (GlcNAc)n, n = 1-6, at initial concentrations as low 10 microM. A modified capillary assay was used quantitation; cells exhibit level constitutive taxis GlcNAc but not oligosaccharides. mutant defective receptor (IINag phosphotransferase system) showed inducible Two (or more) independently receptors with overlapping specificities recognize 2-4. (GlcNAc)5 (GlcNAc)6 were inactive assay; expression this receptor(s) apparently require special induction conditions. 1-4, chemoreceptors may be most potent reported bacteria. L-Amino acids weak, attractants; glutamine, known an attractant other bacteria, effective amino acid. Escherichia coli, Tar (aspartate), expressed furnissii. chemotactic responses greatly affected by growth conditions presence nutrients media. Taxis optimally induced lactate medium containing 0.6 mM sugar, while on sugar per se resulted poor taxis. Chemotaxis sugars increased 2- 3-fold when starved. Nutrients medium, especially compounds that feed into or are part Krebs cycle, inhibitors Gln. With exception isocitrate, inhibition correlated rate oxidation these compounds. results suggest link between catabolism organism, i.e. interactions "cross-talk" systems regulated protein phosphorylation (Stock, Ninfa, A. J., Stock, M. (1989) Microbiol. Rev. 53, 450-490).