Brain-derived neurotrophic factor modulates mitochondrial dynamics and thermogenic phenotype on 3T3-L1 adipocytes.

摘要: Obesity is a growing threat. In recent years, the finding of functional brown adipose tissue (BAT) in adult humans implemented studies anti-obesity therapies based on triggering energy expenditure. The activation BAT thermogenesis and recruitment brite (brown-in-white) adipocytes are under noradrenergic control. Brain-derived neurotrophic factor (BDNF), if centrally administered, enhances through sympathetic activation, but its direct effect still unclear. phenotypic change from fat storing to thermogenic recognized by presence multilocular lipid droplets (LDs) fissed mitochondria that tend surround LDs, maximizing efficiency fatty acid release for thermogenesis. BDNF treatment differentiated 3T3-L1 was compared negative (CTRL) positive (norepinephrine, NE) controls. significantly increased small globular percentage (>150% CTRL), while area surface elongation index branched tubules were respectively 55% 10% lower than NE. Canonical discriminant analysis morphological data clearly separated differentially treated cells with 85% total variance. expression markers mitochondrial dynamic genes affected BDNF. Investigating pathways involved adipocyte stimulation could clarify role possible local regulation.