Direct and simultaneous determination of arsenic, manganese, cobalt and nickel in urine with a multielement graphite furnace atomic absorption spectrometer.

摘要: Abstract A simple method was developed for the direct and simultaneous determination of arsenic (As), manganese (Mn), cobalt (Co), nickel (Ni) in urine by a multi-element graphite furnace atomic absorption spectrometer (Perkin–Elmer SIMAA 6000) equipped with transversely heated atomizer longitudinal Zeeman-effect background correction. Pd used as chemical modifier along either internal gas or containing hydrogen double stage pyrolysis process. standard reference material (SRM) Seronorm™ Trace Elements to confirm accuracy method. The optimum conditions analysis samples are at 1350 °C (using 5% H2 v/v Ar inter during first pure second stage) atomization 2100 °C. use matrix-free standards resulted concentrations all analytes within 85% (As) 110% certified values. recovery As improved when mixture 95% (v/v) applied step two-stage 1350 °C, found values were 91–110% value. recoveries real range 88–95% these four elements. detection limits 0.78 μg l−1 As, 0.054 μg l−1 Mn, 0.22 μg l−1 Co, 0.35 μg l−1 Ni. upper linear calibration curve 60 μg l−1 (As); 12 μg l−1 (Mn); (Co) 25 μg l−1 (Ni), respectively. relative deviations (R.S.D.s) SRM 2% less. R.S.D.s sample 1.6% 6.3% 7.0% 8.0%