Feasibility study on mouse live imaging after spinal cord injury and poly(lactide-co-glycolide) bridge implantation.

摘要: Spinal cord injury (SCI) causes permanent paralysis below the damaged area. SCI is linked to neuronal death, demyelination, and limited ability of fibers regenerate. Regeneration capacity by presence many inhibitory factors in spinal environment. The use poly(lactide-co-glycolide) (PLG) bridges has demonstrated sustain long-term regeneration after a cervical hemisection mouse model. Critically, imaging regenerating myelination status these filaments severe limitation progress research. We used transgenic model that selectively expresses fluorescent reporters (eGFP) cord. implanted PLG bridge at C5 vertebra evaluated live animals' interface within 8 weeks postimplant. These vivo observations were correlated with situ evaluation 12 postimplantation. sectioned cords performed bioimaging on sections observe going through bridge. In parallel, visualize regenerated axons, we exploited characteristics third-harmonic generation arising from myelin structure fixed sections.