Application of the Johnson-Kendall-Roberts model in AFM-based mechanical measurements on cells and gel.
作者： Yu.M. Efremov , D.V. Bagrov , M.P. Kirpichnikov , K.V. Shaitan
DOI: 10.1016/J.COLSURFB.2015.06.044
关键词: Modulus 、 Bovine serum albumin 、 Viscoelasticity 、 Polyacrylamide 、 Elasticity (economics) 、 Composite material 、 Analytical chemistry 、 Force spectroscopy 、 Colloid 、 Indentation 、 Materials science
摘要: The force-distance curves (FCs) obtained by the atomic force microscope (AFM) with colloid probes contain information about both viscoelastic properties and adhesion of a sample. Here, we processed approach retraction parts FCs on polyacrylamide gels (in water or PBS) Vero cells culture medium). Johnson-Kendall-Roberts model was applied to account for adhesion. effects loading rate, holding time indentation depth Young's modulus, calculated from curves, were studied. It shown that bulk local interfacial viscoelasticity can affect observed approach-retraction hysteresis measured parameters. addition 1% bovine serum albumin (BSA) decreased probe PAA gel surface, so diminished. On contrary, adhesiveness increased after BSA addition, indicating complex nature cell-probe interaction.
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