Burkholderia gladioli MB39 an Antarctic Strain as a Biocontrol Agent.

摘要: Bioprospecting sub-explored environments such as Antarctic locations leads to finding out diverse activities, reducing harmful chemical usage that affects both human health and the environment. In this study, ~ 7000 cold-adapted bacterial strains were isolated from samples around Melchior Base at 5 °C more than 13,000 15 °C. Out of them, 900 different colony morphotypes evaluated for antimicrobial production, 13 isolates demonstrated antibacterial antifungal activities. One isolate, closely related Burkholderia gladioli according 16S rDNA (99.8%), gyrB (99.6%) Cpn60 (99.4%) gene sequence analysis, showed a consistent, broad spectrum against pathogenic phytopathogenic bacteria. Its potent activity inhibits growth various plant fungi, whereas it was mainly studied Penicillium digitatum Macrophomina phaseolina, causal agents blue mould in postharvest fruits charcoal rot soybean crops, respectively. The compound exhibited low molecular weight (< 6000 Da), resistance lytic enzymes stability range temperature pHs. Observations B. MB39 effects over M. phaseolina mycelia by scanning electron microscopy alterations hyphal structures, reduced extension, severe cell morphology changes cytoplasmic leakage, flattened empty mycelia. Here we report isolation identification strain. results describe effectiveness antarctic strain fungal phytopathogens biocontrol its potential crop protection plans.