作者: Marta Encheva-Malinova , Mariya Stoyanova , Hristina Avramova , Yanitsa Pavlova , Blagovesta Gocheva
DOI: 10.1080/13102818.2014.947066
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摘要: The exploration of habitats with unusual environment and poorly explored areas such as Antarctica is one the strategies for discovery new biologically active substances and/or producers. aim this study was to identify actinomycetes isolated from soils island Livingston – investigate their potential synthesize antibacterial agents against phytopathogens. Twenty-three actinomycete strains were object study. Using PCR (polymerase chain reaction) amplification all affiliated genus Streptomyces. The sequencing 16S rRNA three showed greatest similarity Streptomyces tendae them, revealed that other had closest relations streptomycetes anthropogenically unaltered regions including Antarctica. The isolates studied production both by molecular culture methods. targeting specific biosynthetic genes involved in some groups antibiotics performed. screening possessed gene Type-II polyketide synthase, 11 non-ribosomal peptide synthetase; 6 polyene antibiotics; 4 glycopeptide antibiotics. tested vitro phytopathogenic bacteria. differed number inhibited test bacteria spectrum action. Four a wide range activity Gram-positive Gram-negative phytopathogens. The results obtained Antarctic are source isolation producing different groups.