Trypanosoma cruzi: Analysis of the Population Dynamics of Heterogeneous Mixtures1
作者： Richard W. Finley , James A. Dvorak
DOI: 10.1111/J.1550-7408.1987.TB03202.X
关键词: Flow cytometry 、 Immunology 、 Population density 、 Biology 、 Parasite hosting 、 Protozoa 、 Trypanosoma cruzi 、 Population 、 Clone (cell biology) 、 Exponential growth 、 Genetics
摘要: Utilizing the previously reported inter-clonal differences in total DNA/organism, flow cytometry was used to analyze population dynamics of Trypanosoma cruzi clone mixtures growing liquid medium or vertebrate cells. The growth can be described by unique parameters reflecting exponential rate (r), stationary phase density (1/k), and interaction between clones (h). relative numbers each change rapidly with time results arc quantitative agreement mathematical models competitive growth. relationship for T. is such that, general, there no dynamic equilibrium coexistence different rates; under all culture protocols, faster will prevail. A computer simulation cell cycle suggests that grow relatively independently; basic attributes model were substantiated experimentally. Although wide fluctuations proportion released occurred, again predominated. Finally, these underline importance working well-defined laboratory avoid inconsistencies paradoxical stress rapid isolation single from clinical specimens when studying parasite human disease.
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