Rapid synthesis of silver nanoparticles by Pseudomonas stutzeri isolated from textile soil under optimised conditions and evaluation of their antimicrobial and cytotoxicity properties.
作者： Nishant Rajora , Sanket Kaushik , Anupam Jyoti , Shanker L. Kothari
DOI: 10.1049/IET-NBT.2015.0107
关键词: Absorbance 、 Pseudomonas stutzeri 、 Microbiology 、 Chemistry 、 Fourier transform infrared spectroscopy 、 Antimicrobial 、 Nuclear chemistry 、 Escherichia coli 、 Ultraviolet visible spectroscopy 、 Silver nanoparticle 、 Antibacterial activity
摘要: Present study utilised textile soil isolated bacterium Pseudomonas stutzeri to synthesise extracellular silver nanoparticles (AgNPs) under optimised conditions. The synthesised AgNPs were characterised using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Optimisation showed synthesis within 8 h 2mM Ag nitrate at pH9, temperature 80°C maximum absorbance toward 400 nm. TEM analysis revealed spherical shape reduction in size upto nm was observed FTIR spectra confirmed presence of proteins bound act as reducing agent. strong antibacterial activity against multi-drug resistant (MDR) Escherichia coli Klebsiella pneumoniae demonstrated by disc diffusion colony forming unit assays. Zone inhibition increased with increasing concentration 19 mm E. 17 K. 2 μg/disc. Furthermore, did not show any cytotoxic effects on human epithelial cells 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay even μg/ml AgNPs. results the present suggest that can be rapidly conditions antimicrobial property MDR pathogens without having toxicity effect cells.
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