Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
作者： Susana Fernandes-Silva , Joana Moreira-Silva , Tiago H. Silva , Ricardo I. Perez-Martin , Carmen G. Sotelo
关键词: Composite material 、 Self-healing hydrogels 、 Tissue engineering 、 Carbon dioxide in Earth's atmosphere 、 In vitro cell culture 、 Supercritical fluid 、 Genipin 、 Cell culture 、 Materials science 、 Porosity
摘要: The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin used prepare pre-scaffolds by freeze-drying. poor stability of the and low mechanical properties require crosslinking structures. Under dense CO2 atmosphere, allowed for 16 h. Additionally, hydrogels are foamed scaffolds obtained present a highly structure. In vitro cell culture tests performed chondrocyte-like line show good adherence proliferation, which strong indication potential these be in tissue cartilage engineering.
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