Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling
作者： Richard H. Perry , David I. Bellovin , Emelyn H. Shroff , Ali I. Ismail , Tahera Zabuawala
DOI: 10.1021/AC400479J
关键词: Chemistry 、 Lipid metabolism 、 Proto-Oncogene Proteins c-myc 、 Genetically modified organism 、 Oncogene 、 Genetically modified mouse 、 Cell biology 、 Molecular biology 、 Gene 、 Carcinogenesis 、 In situ
摘要: We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach combining DESI-MSI with the ability turn specific genes on and off has led discovery highly lipid molecules associated tumor onset. are able distinguish normal malignant cells. Our provides strategy define precise molecular picture at resolution about 200 μm that may be useful identifying how MYC oncogene initiates maintains tumorigenesis.
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