Activation of benzo[a]pyrene and 2-aminoanthracene to bacteria mutagens by mussel digestive gland postmitochondrial fraction
作者： X.R. Michael , P.M. Cassand , J.F. Narbonne
DOI: 10.1016/0165-7992(93)90033-R
关键词: Biochemistry 、 Monooxygenase 、 Chemistry 、 Benzo(a)pyrene 、 Amine oxidase 、 Cytochrome 、 Mussel 、 Aromatic amine 、 Carcinogen 、 Pyrene 、 General Medicine
摘要: Abstract The ability of the mussel postmitochondrial fraction (S9) to activate benzo[a]pyrene (BaP) and 2-aminoanthracene (2AA) mutagenic metabolites towards Salmonella typhimurium strain TA98 was tested. mechanisms involved in this activation were investigated cytochrome P-450-dependent monooxygenases its NADPH c reductase found contribute BaP. This improved by treating with 4,5,4′,5′-tetrachlorobiphenyl (TCB) (a 3-methylcholanthrene-type inducer monooxygenase marine fish) inhibited α-naphthoflavone (ANF), a P-450 inhibitor. However, both BaP cytchrome P-450-related metabolic activities are much weaker mussels than vertebrates. Mussel S9 activates aromatic amines more effectively Pretreatment TCB or addition ANF incubation medium has no effect on 2AA activation. As suggested Kurelec (1985), amine metabolism may be supported flavoprotein mixed-function oxidase which is NADPH-dependent.
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