In Vitro Detection and Quantification of Testosterone Metabolites in Aquatic Organisms
作者： Nadia Aarab , Pascal Mora , Michèle Daubèze , Jean‐François Narbonne
DOI: 10.1081/AL-200050309
关键词: Metabolite 、 Corbicula fluminea 、 Biochemistry 、 Trout 、 Aquatic animal 、 Mytilus 、 Sentinel species 、 Androstenedione 、 Chemistry 、 Environmental pollution 、 Environmental chemistry
摘要: Abstract High‐performance liquid chromatography (HPLC) was used to evaluate testosterone metabolism in aquatic animals generally as sentinel species (i.e., fish and molluscs). The profiles of metabolites resulting from incubation liver microsomes trout Oncorhynchus mykiss, digestive gland S9 clams, Corbicula fluminea mussels Mytilus galloprovincialis were compared. Main 6β‐hydroxytestosterone, 16α‐hydroxytestosterone, 11β‐hydroxytestosterone, 2α‐hydroxytestosterone, 2β‐hydroxytestosterone androstenedione detected quantified rat, fish, clam, whereas only 16α‐hydroxytestosterone mussels. Testosterone metabolite cellular fractions further analyzed. protocol presented this study may be considered a useful tool for biomarker based pollution surveys. Supported by the European project Biological Effects Environmental Pollution marine coastal ecosystems: BEE...
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