Characterization of Plasmodium berghei antigens inducing blast transformation in immune rat lymphocytes.
作者： Eduardo A. Nillni , D. T. Spira
DOI: 10.1007/BF00927402
关键词: Biology 、 Immune system 、 Size-exclusion chromatography 、 Salting out 、 Ion chromatography 、 Centrifugation 、 Antigen 、 Plasmodium berghei 、 Molecular mass 、 Molecular biology
摘要: Rat erythrocytes, infected withPlasmodium berghei, were disrupted by freezing and thawing or parasites released mechanical breaking of the agglutinated erythrocytes. The soluble antigens, inducing blast transformation in non-adherent immune rat spleen cells, fractionated a sequence salting out, ion exchange chromatography gel filtration. At final step purification factor 83.7-fold was achieved. This fraction contained three components discernible SDS-PAGE molecular weights 56, 66 72 kD respectively. free parasites, also induced cells. After disruption freeze-thawing majority active antigens found sediment after centrifugation at 17,300g.
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