Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection Pressure

摘要: 5-Fluorouracil (5-FU) remains the gold standard of first-line treatment for colorectal cancer (CRC). Although it may initially debulk tumor mass, relapses frequently occur, indicating existence cells that are therapy-resistant and capable refueling growth. To identify mechanisms drug resistance, CRC stem-like were subjected to long-term 5-FU selection using either intermittent regimen with IC50 dose or continuous escalating doses. Parental cultivated in parallel. Real-time PCR arrays bioinformatic tools used investigate gene expression changes. We found first method selected more aggressive features. therefore transplanted these parental mice, again, not only did 5-FU-selected generate tumors respect their counterpart, but they also showed a different pattern as compared what we had observed vitro, ID1 top upregulated gene. propose stemness marker pervasively expressed secondary lesions emerging after completion chemotherapy.