Cdc25A is a novel phosphatase functioning early in the cell cycle

摘要: The cdc25+ tyrosine phosphatase is a key mitotic inducer of the fission yeast Schizosaccharomyces pombe, controlling timing initiation mitosis. Mammals contain at least three homologues called cdc25A, cdc25B and cdc25C. In this study we investigate biological function cdc25A. Although very potent in rescuing S.pombe cdc25 mutant, cdc25A less structurally related to enzyme. Northern Western blotting detection reveals that unlike cdc25B, cdc25C cdc2, predominantly expressed late G1. Moreover, immunodepletion rat cells by microinjection specific antibody effectively blocks their cell cycle progression from G1 into S phase, as determined laser scanning single cytometry. These results indicate not regulator but novel plays crucial role start cycle. view its strong ability activate cdc2 kinase expression G1, cdc2-related kinases functioning early may be targets for phosphatase.