Rapid quantitative detection of capsaicinoids in serum based on an electrochemical immunosensor with a dual-signal amplification strategy

摘要: In this paper, a dual-signal amplified electrochemical immunosensor for quantitative detection of capsaicinoids in serum was reported, which could be used to study the pharmacological properties capsaicinoids. To improve sensitivity immunosensor, glassy carbon electrode modified with Prussian blue-gold nanocomposites (PB-AuNPs) and thionine (TH). PB-AuNPs provided better electrical conductivity. At same time, TH enhanced stability electron transfer, leading superior performance. Meanwhile, an excellent anti-capsaicinoids antibody (Ab) ensure specificity immunosensor. The prepared tested using cyclic voltammetry (CV) differential pulse (DPV). Under optimal conditions, proposed displayed wide linear range from 0.01 1000 ng/mL limit 0.01 ng/mL showed good recoveries (85.12–104.25%) high stability. Additionally, successfully applied monitor real samples.