In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes

摘要: The sequencing of 16S and 23S ribosomal RNA (rRNA) molecules is currently the gold standard for classification new microbial isolates. Comparative analyses these sequences are first time in history microbiology facilitating reconstruction universal phylogenetic trees [38]. Among many other important findings work Carl Woese his colleagues demonstrated that only certain (by far not all) phenotypic/physiological groups micro-organisms monophyletic (e.g., methanogenes, cyanobacteria, spirochetes). About 10 years ago it has been proposed to use an rRNA approach studies ecology [21]. diversity should be analyzed a cultivation-independent way by direct sequence retrieval, whereas nucleic acid probes complementary or genes tools monitor population dynamics environmental samples. By their own nature rRNA-targeted track genotypes which necessarily linked one phenotype. Microbial ecologists who want apply this investigate correlations between community structures functions aware fact design accordingly.