CIRP regulates BEV-induced cell migration in gliomas.

作者: Yu-Xiao Liu , Jun-Nian Zhou , Ke-Hui Liu , Xiang-pin Fu , Zhi-Wen Zhang

DOI: 10.2147/CMAR.S191249

关键词: Cell migrationGliomaProteomicsEctopic expressionImmunoprecipitationMicroarray analysis techniquesChemistryTransport proteinCancer researchCell adhesion

摘要: Purpose A better understanding of the underlying molecular mechanisms in treatment failure bevacizumab (BEV) for malignant glioma would contribute to overcome therapeutic resistance. Methods Here, we used a quantitative proteomic method identify signatures glioblastoma cell after BEV by two-dimensional liquid chromatography-tandem mass spectrometry analysis and 6-plex iTRAQ quantification. Next, function cold-inducible RNA-binding protein (CIRP), one most significantly affected proteins drug treatment, was evaluated resistance cells invasion assays animal xenograft assays. Target molecules bound CIRP were determined using immunoprecipitation microarray analysis. Then, these mRNAs identified real-time PCR. Results Eighty-seven with significant fold changes. The biological functional indicated that involved process cellular signal transduction, adhesion, transport. expression greatly decreased ectopic abolished migration BEV-treated cells. In addition, could bind mRNA CXCL12 inhibit BEV-induced increase Conclusion These data suggested may take part gliomas binding migration-relative RNAs.

参考文章(27)
Regina Fillbrandt, Katrin Lamszus, Ulrike Ulbricht, Philip Kunkel, Manfred Westphal, Dimitrios Stavrou, Marc A. Brockmann, Peter Bohlen, Inhibition of glioma angiogenesis and growth in vivo by systemic treatment with a monoclonal antibody against vascular endothelial growth factor receptor-2. Cancer Research. ,vol. 61, pp. 6624- 6628 ,(2001)
W. Tang, X. Wang, Y. Chen, J. Zhang, Y. Chen, Z. Lin, CXCL12 and CXCR4 as predictive biomarkers of glioma recurrence pattern after total resection. Pathologie Biologie. ,vol. 63, pp. 190- 198 ,(2015) , 10.1016/J.PATBIO.2015.07.002
Baharak Vafadar-Isfahani, Graham Ball, Clare Coveney, Christophe Lemetre, David Boocock, Lennart Minthon, Oskar Hansson, Amanda Kathleen Miles, Sabina M Janciauskiene, Donald Warden, A. David Smith, Gordon Wilcock, Noor Kalsheker, Robert Rees, Balwir Matharoo-Ball, Kevin Morgan, Identification of SPARC-like 1 Protein as Part of a Biomarker Panel for Alzheimer's Disease in Cerebrospinal Fluid Journal of Alzheimer's Disease. ,vol. 28, pp. 625- 636 ,(2012) , 10.3233/JAD-2011-111505
C. Burd, G Dreyfuss, Conserved structures and diversity of functions of RNA-binding proteins. Science. ,vol. 265, pp. 615- 621 ,(1994) , 10.1126/SCIENCE.8036511
Antje Wick, Nils Dörner, Navina Schäfer, Silvia Hofer, Sabine Heiland, Daniela Schemmer, Michael Platten, Michael Weller, Martin Bendszus, Wolfgang Wick, Bevacizumab does not increase the risk of remote relapse in malignant glioma. Annals of Neurology. ,vol. 69, pp. 586- 592 ,(2011) , 10.1002/ANA.22336
Rakesh K. Jain, Emmanuelle di Tomaso, Dan G. Duda, Jay S. Loeffler, A. Gregory Sorensen, Tracy T. Batchelor, Angiogenesis in brain tumours Nature Reviews Neuroscience. ,vol. 8, pp. 610- 622 ,(2007) , 10.1038/NRN2175
Diane Bergeron, Diane Beauseigle, Guy Bellemare, Sequence and expression of a gene encoding a protein with RNA-binding and glycine-rich domains in Brassica napus Biochimica et Biophysica Acta. ,vol. 1216, pp. 123- 125 ,(1993) , 10.1016/0167-4781(93)90047-H
Zhiping Xia, Xinmin Zheng, Hang Zheng, Xiaojun Liu, Zhonghua Yang, Xinghuan Wang, Cold-inducible RNA-binding protein (CIRP) regulates target mRNA stabilization in the mouse testis FEBS Letters. ,vol. 586, pp. 3299- 3308 ,(2012) , 10.1016/J.FEBSLET.2012.07.004
Karl H. Plate, Alexander Scholz, Daniel J. Dumont, Tumor angiogenesis and anti-angiogenic therapy in malignant gliomas revisited Acta Neuropathologica. ,vol. 124, pp. 763- 775 ,(2012) , 10.1007/S00401-012-1066-5
Yu Zeng, Prakash Kulkarni, Takahiro Inoue, Robert H. Getzenberg, Down-regulating cold shock protein genes impairs cancer cell survival and enhances chemosensitivity. Journal of Cellular Biochemistry. ,vol. 107, pp. 179- 188 ,(2009) , 10.1002/JCB.22114