MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae.

摘要: Pheromone-stimulated yeast cells and haploid gpa1 deletion mutants arrest their cell cycle in G1. Overexpression of a novel gene called MSG5 suppresses this inhibition division. Loss function leads to diminished adaptive response pheromone. Genetic analysis indicates that acts at stage where the protein kinases STE7 FUS3 transmit pheromone-induced signal. Since loss causes an increase enzyme activity but not activity, we propose impinges on pathway FUS3. Sequence suggests encodes tyrosine phosphatase. This is supported by finding recombinant has phosphatase vitro able inactivate autophosphorylated Thus might stimulate recovery from pheromone regulating phosphorylation state