Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin.
作者： M.A. Menezes , L.B. Rocha , P.C.M. Koga , I. Fernandes , J.M. Nara
DOI: 10.1111/J.1365-2672.2009.04484.X
关键词: Monoclonal antibody 、 Enteropathogenic Escherichia coli 、 Escherichia coli 、 Antiserum 、 Serotype 、 Polyclonal antibodies 、 Biology 、 Intimin 、 Enterobacteriaceae 、 Microbiology
摘要: Aims: To evaluate the sensitivity and specificity of polyclonal monoclonal antibodies (Mabs) against intimin in detection enteropathogenic enterohaemorrhagic Escherichia coli isolates using immunoblotting. Methods Results: Polyclonal Mabs intimin-conserved region were raised, their reactivities compared E. coli (EPEC) (EHEC) immunoblotting analysis. In comparison with rat antiserum, rabbit anti-intimin IgG-enriched fraction had a stronger recognition pattern to wide spectrum types different EPEC EHEC serotypes. On other hand, murine IgG2b specific intimin, dissociation constant 1·3 × 10−8 mol l−1, failed some these isolates. Conclusion: All employed showed 100% specificity, not reacting any eae-negative isolates. The range was according antisera, 97% for fraction, followed by 92% 78% antisera Mab. Significance Impact Study: analysis is useful tool diagnoses.
core.ac.uk
cabdirect.org
fapesp.br
researchgate.net 
sci-hub.se 参考文章(78)
Mannu F, Colombo Mm, Cappuccinelli P, Fiori Pl, Rappelli P, Maddau G, Development of a set of multiplex PCR assays for the simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. New Microbiologica. ,vol. 24, pp. 77- 83 ,(2001)
Miguel Blanco, Sandra Schumacher, Taurai Tasara, Claudio Zweifel, Jesús E Blanco, Ghizlane Dahbi, Jorge Blanco, Roger Stephan, Serotypes, intimin variants and other virulence factors of eae positive Escherichia coli strains isolated from healthy cattle in Switzerland. Identification of a new intimin variant gene (eae-η2) BMC Microbiology. ,vol. 5, pp. 23- 23 ,(2005) , 10.1186/1471-2180-5-23
John R. Crowther, The ELISA Guidebook ,(2000)
G Frankel, D C Candy, P Everest, G Dougan, Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei. Infection and Immunity. ,vol. 62, pp. 1835- 1842 ,(1994) , 10.1128/IAI.62.5.1835-1842.1994
Jeannette Adu-Bobie, Gad Frankel, Christopher Bain, Azizedite Guedes Goncalves, Luiz R. Trabulsi, Gill Douce, Stuart Knutton, Gordon Dougan, Detection of Intimins α, β, γ, and δ, Four Intimin Derivatives Expressed by Attaching and Effacing Microbial Pathogens Journal of Clinical Microbiology. ,vol. 36, pp. 662- 668 ,(1998) , 10.1128/JCM.36.3.662-668.1998
J Franke, S Franke, H Schmidt, A Schwarzkopf, L H Wieler, G Baljer, L Beutin, H Karch, Nucleotide sequence analysis of enteropathogenic Escherichia coli (EPEC) adherence factor probe and development of PCR for rapid detection of EPEC harboring virulence plasmids. Journal of Clinical Microbiology. ,vol. 32, pp. 2460- 2463 ,(1994) , 10.1128/JCM.32.10.2460-2463.1994
Adrienne W. Paton, James C. Paton, Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157 Journal of Clinical Microbiology. ,vol. 36, pp. 598- 602 ,(1998) , 10.1128/JCM.36.2.598-602.1998
Sean D. Reid, David J. Betting, Thomas S. Whittam, Molecular Detection and Identification of Intimin Alleles in Pathogenic Escherichia coli by Multiplex PCR Journal of Clinical Microbiology. ,vol. 37, pp. 2719- 2722 ,(1999) , 10.1128/JCM.37.8.2719-2722.1999
S. Persson, K.E.P. Olsen, F. Scheutz, K.A. Krogfelt, P. Gerner-Smidt, A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory Clinical Microbiology and Infection. ,vol. 13, pp. 516- 524 ,(2007) , 10.1111/J.1469-0691.2007.01692.X
M S Donnenberg, J B Kaper, Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infection and Immunity. ,vol. 59, pp. 4310- 4317 ,(1991) , 10.1128/IAI.59.12.4310-4317.1991