Protist Diversity and Seasonal Dynamics in Skagerrak Plankton Communities as Revealed by Metabarcoding and Microscopy.
作者： Sandra Gran‐Stadniczeñko , Elianne Egge , Vladyslava Hostyeva , Ramiro Logares , Wenche Eikrem
DOI: 10.1111/JEU.12700
关键词: Biology 、 Photic zone 、 Species richness 、 Phytoplankton 、 Plankton 、 Abundance (ecology) 、 Zooplankton 、 Protist 、 Trophic level 、 Ecology
摘要: Protist community composition and seasonal dynamics are of major importance for the production higher trophic levels, such as zooplankton fish. Our aim was to reveal how protist in Skagerrak changes through seasons by combining high-throughput sequencing microscopy plankton collected monthly over two years. The V4 region 18S rRNA gene amplified eukaryote universal primers from total RNA/cDNA. We found a strong variation proportional abundances, difference between depths within euphotic zone. Highest richness late summer-early autumn, lowest winter. Temperature abiotic factor explaining most diversity. Dinoflagellates abundant diverse group followed ciliates diatoms. about 70 new taxa recorded first time Skagerrak. pattern relative read abundance phytoplankton groups well accordance with microscopical biovolumes. This is metabarcoding study all taxonomic Skagerrak, which may serve baseline future surveys effects climate environmental changes.
sci-hub.se 参考文章(66)
Fabrice Not, Raffaele Siano, Wiebe H.C.F. Kooistra, Nathalie Simon, Daniel Vaulot, Ian Probert, Diversity and Ecology of Eukaryotic Marine Phytoplankton Advances in Botanical Research. ,vol. 64, pp. 1- 53 ,(2012) , 10.1016/B978-0-12-391499-6.00001-3
Kazutaka Katoh, George Asimenos, Hiroyuki Toh, Multiple Alignment of DNA Sequences with MAFFT Methods in Molecular Biology. ,vol. 537, pp. 39- 64 ,(2009) , 10.1007/978-1-59745-251-9_3
David M. John, Alan J. Brook., Brian A. Whitton, The freshwater algal flora of the British Isles : anidentification guide to freshwater and terrestrial algae Cambridge University Press. ,(2011)
Purificación López-García, Francisco Rodríguez-Valera, Carlos Pedrós-Alió, David Moreira, Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton. Nature. ,vol. 409, pp. 603- 607 ,(2001) , 10.1038/35054537
Ramon Massana, Protistan Diversity in Environmental Molecular Surveys Springer, Tokyo. pp. 3- 21 ,(2015) , 10.1007/978-4-431-55130-0_1
Colomban De Vargas, Stéphane Audic, Nicolas Henry, Johan Decelle, Frédéric Mahé, Ramiro Logares, Enrique Lara, Cédric Berney, Noan Le Bescot, Ian Probert, Margaux Carmichael, Julie Poulain, Sarah Romac, Sébastien Colin, Jean-Marc Aury, Lucie Bittner, Samuel Chaffron, Micah Dunthorn, Stefan Engelen, Olga Flegontova, Lionel Guidi, Aleš Horák, Olivier Jaillon, Gipsi Lima-Mendez, Julius Lukeš, Shruti Malviya, Raphael Morard, Matthieu Mulot, Eleonora Scalco, Raffaele Siano, Flora Vincent, Adriana Zingone, Céline Dimier, Marc Picheral, Sarah Searson, Stefanie Kandels-Lewis, Tara Oceans Coordinators, Silvia G Acinas, Peer Bork, Chris Bowler, Gabriel Gorsky, Nigel Grimsley, Pascal Hingamp, Daniele Iudicone, Fabrice Not, Hiroyuki Ogata, Stephane Pesant, Jeroen Raes, Michael E Sieracki, Sabrina Speich, Lars Stemmann, Shinichi Sunagawa, Jean Weissenbach, Patrick Wincker, Eric Karsenti, Emmanuel Boss, Michael Follows, Lee Karp-Boss, Uros Krzic, Emmanuel G Reynaud, Christian Sardet, Matthew B Sullivan, Didier Velayoudon, Eukaryotic plankton diversity in the sunlit ocean Science. ,vol. 348, pp. 1261605- 1261605 ,(2015) , 10.1126/SCIENCE.1261605
Elianne Egge, Lucie Bittner, Tom Andersen, Stéphane Audic, Colomban de Vargas, Bente Edvardsen, 454 Pyrosequencing to Describe Microbial Eukaryotic Community Composition, Diversity and Relative Abundance: A Test for Marine Haptophytes PLoS ONE. ,vol. 8, pp. e74371- 15 ,(2013) , 10.1371/JOURNAL.PONE.0074371
Deepak Nanjappa, Wiebe H. C. F. Kooistra, Adriana Zingone, A reappraisal of the genus Leptocylindrus (Bacillariophyta), with the addition of three species and the erection of Tenuicylindrus gen. nov. Journal of Phycology. ,vol. 49, pp. 917- 936 ,(2013) , 10.1111/JPY.12102
PETER D. COUNTWAY, REBECCA J. GAST, PRATIK SAVAI, DAVID A. CARON, Protistan diversity estimates based on 18S rDNA from seawater incubations in the Western North Atlantic. Journal of Eukaryotic Microbiology. ,vol. 52, pp. 95- 106 ,(2005) , 10.1111/J.1550-7408.2005.05202006.X
M. Kuylenstierna, B. Karlson, Seasonality and Composition of Pico- and Nanoplanktonic Cyanobacteria and Protists in the Skagerrak Botanica Marina. ,vol. 37, pp. 17- 34 ,(1994) , 10.1515/BOTM.1994.37.1.17