Rapid identification and typing of Yersinia pestis and other Yersinia species by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry

摘要: Accurate identification is necessary to discriminate harmless environmental Yersinia species from the food-borne pathogens enterocolitica and pseudotuberculosis group A bioterrorism plague agent pestis. In order circumvent limitations of current phenotypic PCR-based methods, we aimed assess usefulness matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) protein profiling for accurate rapid species. As a first step, built database 39 different strains representing 12 species, including 13 Y. pestis isolates representative Antiqua, Medievalis Orientalis biotypes. The organisms were deposited on MALDI-TOF plate after appropriate ethanol-based inactivation, profile was obtained within 6 minutes each When compared with 3,025-profile database, every yielded unique unambiguously identified. second step analysis, clinical (n = 2) 11) correctly particular, identified at level, able successfully differentiate three These data indicate that can be used as first-line method isolates.