Enzyme histochemistry of rat mast cell tryptase.

摘要: Fixation and staining conditions for rat mast cell tryptase its histochemical distribution in different tissues were investigated. Prostate, skin, lung, gut, stomach salivary glands fixed either aldehyde or Carnoy fixatives then frozen embedded paraffin wax. Preservation of enzymic activity against peptide substrates required fixation sectioning. Of the examined, z-Ala-Ala-Lys-4-methoxy-2-naphthylamide z-Gly-Pro-Arg-4-methoxy-2-naphthylamide proved most effective demonstration tryptase. Double by enzyme cytochemistry followed immunological detection showed that, all tryptase-containing cells, is at least part active. Conventional dye-binding histochemistry was used to confirm identity cells. Aldehyde-fixed mucosal cells a much shorter time with Toluidine Blue if tissue sections washed directly t-butyl alcohol. dye binding that absent from subepidermal which are also smaller size appear contain fewer granules than connective This study demonstrates tryptase, unlike tryptases other species, soluble enzyme. It stored an active form some subpopulations mucosa, skin lung. © 1998 Chapman & Hall