Flow cytometry using paraffin-embedded tissue: Five years on

摘要: The use of paraffin-embedded tissue for flow cytometry is reviewed. A number technical modifications the original 1983 method have been described, aimed at improving accuracy DNA measurements by minimizing cell debris or reducing coefficients variation, and simplifying sample preparation. Over 100 clinical studies now reported, mainly assessing effect index on prognosis, those published up until mid-1988 are summarized in an appendix. More recently there developments monoclonal antibodies to measure oncogene products proliferation markers addition content. Detailed evaluation standardization these more sophisticated methods still some way ahead, but as was case with index, archival material from patients whose outcome already known should speed this process.