A radial calibration window for analytical ultracentrifugation
作者： Thomas LeBrun , Peter Schuck , Ren Wei , Justine S. Yoon , Xianghui Dong
DOI: 10.1371/JOURNAL.PONE.0201529
关键词: Physics 、 Particle size 、 Analytical Ultracentrifugation 、 Scale (ratio) 、 Calibration 、 Computational physics 、 Artifact (error) 、 Reticle 、 Magnification 、 Residual
摘要: Analytical ultracentrifugation (AUC) is a first-principles based method for studying macromolecules and particles in solution by monitoring the evolution of their radial concentration distribution as function time presence high centrifugal field. In sedimentation velocity experiments, hydrodynamic properties relating to size, shape, density, solvation can be measured, at resolution, on polydisperse samples. recent multilaboratory benchmark study including data from commercial analytical ultracentrifuges 67 laboratories, calibration accuracy dimension was found one dominant factors limiting AUC. present work, we develop an artifact consisting accurately calibrated reflective pattern lithographically deposited onto AUC window. It serves reticle when scanned control experiments absolute magnification. After analysis pitch between landmarks scans using different optical systems, estimate that residual uncertainty magnification after external with scale ≈0.2 %, similar magnitude other important contributions such temperature time. The previous had many instruments errors measurements 1 % 2 few excess 15 meaning use developed here could reduce 5-to 10-fold or more. Adoption thus factor assuring studies related molecular hydrodynamics particle size
harvard.edu
sci-hub.se 参考文章(32)
Steven A. Berkowitz, John S. Philo, Characterizing Biopharmaceuticals using Analytical Ultracentrifugation Biophysical Characterization of Proteins in Developing Biopharmaceuticals. pp. 211- 260 ,(2015) , 10.1016/B978-0-444-59573-7.00009-9
Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L Bain, Marina M Bakhtina, Donald F Becker, Gregory J Bedwell, Ahmet Bekdemir, Tabot MD Besong, Catherine Birck, Chad A Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B Chaires, Catherine T Chaton, Helmut Cölfen, Keith D Connaghan, Kimberly A Crowley, Ute Curth, Tina Daviter, William L Dean, Ana I Díez, Christine Ebel, Debra M Eckert, Leslie E Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A Fagan, Robert Fairman, Ron M Finn, Wolfgang Fischle, José García De La Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E Harding, José G Hernández Cifre, Andrew B Herr, Elizabeth E Howell, Richard S Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A Kusznir, Hyewon Kwon, Adam Larson, Thomas M Laue, Aline Le Roy, Andrew P Leech, Hauke Lilie, Karolin Luger, Juan R Luque-Ortega, Jia Ma, Carrie A May, Ernest L Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Mücke, Luitgard Nagel-Steger, Geeta J Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K Park, Jin-Ku Park, Peter D Pawelek, Erby E Perdue, Stephen J Perkins, Matthew A Perugini, Craig L Peterson, Martin G Peverelli, Grzegorz Piszczek, Gali Prag, Peter E Prevelige, Bertrand DE Raynal, Lenka Rezabkova, Klaus Richter, Alison E Ringel, Rose Rosenberg, Arthur J Rowe, Arne C Rufer, David J Scott, Javier G Seravalli, Alexandra S Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M Strauss, Werner W Streicher, John P Sumida, Sarah G Swygert, Roman H Szczepanowski, Ingrid Tessmer, Ronald T Toth IV, Ashutosh Tripathy, Susumu Uchiyama, Stephan FW Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H Von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M Wubben, Peter Schuck, None, A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation PLOS ONE. ,vol. 10, pp. e0126420- ,(2015) , 10.1371/JOURNAL.PONE.0126420
Neil Errington, Arthur J. Rowe, Probing conformation and conformational change in proteins is optimally undertaken in relative mode European Biophysics Journal. ,vol. 32, pp. 511- 517 ,(2003) , 10.1007/S00249-003-0315-X
Stephen J. Perkins, Ruodan Nan, Keying Li, Sanaullah Khan, Yuki Abe, Analytical ultracentrifugation combined with X-ray and neutron scattering: Experiment and modelling. Methods. ,vol. 54, pp. 181- 199 ,(2011) , 10.1016/J.YMETH.2011.01.004
Walter Mächtle, HIGH-RESOLUTION, SUBMICRON PARTICLE SIZE DISTRIBUTION ANALYSIS USING GRAVITATIONAL-SWEEP SEDIMENTATION Biophysical Journal. ,vol. 76, pp. 1080- 1091 ,(1999) , 10.1016/S0006-3495(99)77273-5
Patrick H. Brown, Andrea Balbo, Peter Schuck, On the analysis of sedimentation velocity in the study of protein complexes European Biophysics Journal. ,vol. 38, pp. 1079- 1099 ,(2009) , 10.1007/S00249-009-0514-1
Rodolfo Ghirlando, Andrea Balbo, Grzegorz Piszczek, Patrick H. Brown, Marc S. Lewis, Chad A. Brautigam, Peter Schuck, Huaying Zhao, Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references Analytical Biochemistry. ,vol. 440, pp. 81- 95 ,(2013) , 10.1016/J.AB.2013.05.011
Jeffrey A. Fagan, Ming Zheng, Vinayak Rastogi, Jeffrey R. Simpson, Constantine Y. Khripin, Carlos A. Silvera Batista, Angela R. Hight Walker, Analyzing surfactant structures on length and chirality resolved (6,5) single-wall carbon nanotubes by analytical ultracentrifugation. ACS Nano. ,vol. 7, pp. 3373- 3387 ,(2013) , 10.1021/NN4002165
Sergio R. Aragon, Recent advances in macromolecular hydrodynamic modeling. Methods. ,vol. 54, pp. 101- 114 ,(2011) , 10.1016/J.YMETH.2010.10.005
Rodolfo Ghirlando, Huaying Zhao, Andrea Balbo, Grzegorz Piszczek, Ute Curth, Chad A. Brautigam, Peter Schuck, Measurement of the temperature of the resting rotor in analytical ultracentrifugation Analytical Biochemistry. ,vol. 458, pp. 37- 39 ,(2014) , 10.1016/J.AB.2014.04.029