Probing conformation and conformational change in proteins is optimally undertaken in relative mode

摘要: Hydrodynamic bead modelling has been widely used in attempts to assess the 3D conformation of proteins solution. Initially, simple models employing only a small number beads were used, with considerable degree success. Latterly, high-resolution based upon atomic coordinates have developed, and much more sophisticated questions can principle be addressed. A detailed analysis is presented errors involved generation such associated prediction (translational friction) parameters, practical measurement these parameters for comparison. It shown that most cases, particle moderate asymmetry, are it not feasible determine, on an absolute basis, which range candidate conformers "correct" one. However, when properties compared relation those "paradigm conformer", whose structure solution, basis external evidence, accepted as correct, then cancel very precise comparisons become possible. The (and hence data files) matter, using existing program MacBEADS, further facilitated by display module (pro Fit).