Measurement of the temperature of the resting rotor in analytical ultracentrifugation
作者： Rodolfo Ghirlando , Huaying Zhao , Andrea Balbo , Grzegorz Piszczek , Ute Curth
关键词: Analytical Ultracentrifugation 、 Rotor (electric) 、 Mechanics 、 Analytical chemistry 、 Ultra-high vacuum 、 Calibration 、 Spinning 、 Thermal equilibration 、 Chemistry
摘要: Accurate measurements of rotor temperature are critical for the interpretation hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods a more accurate determination spinning using iButton loggers. Here we report that measured with on counterbalance resting rotor, following thermal equilibration under high vacuum, closely corresponded to precision better than 0.2°C. This strategy offers an inexpensive and straightforward approach monitor accuracy calibration determine corrective offsets.
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