Mutagenic effect of BUdR in diploid human fibroblasts.

摘要: Abstract It has only recently been possible to demonstrate the expected mutagenic effect of 5-bromodeoxyuridine (BUdR) in heteroploid hamster cells culture. We have now extended this observation diploid human fibroblasts utilizing techniques adapted from work Albertini and DeMars on X-tay mutagenesis at hypoxanthine-guanine phosphoribosyltransferase (HGPRT) locus these cells. In four separate experiments, a female donor were exposed 500 μg/ml ethylmethane sulfonate (EMS) or 3 BUdR yielding survivals 9% 5%, respectively. After 6-day expression period, survivors plated selection medium containing 0.3 8-azaguanine (8-AG). 3–5 weeks, azaguanine-resistant colonies isolated for characterization stained counting. The average spontaneous mutation rate/cell/generation was 0.6·10 −6 . induced rates EMS 7.8·10 6.3·10 /cell/generation, Similar results obtained two experiments with an additional fibroblast line. Mutant following treatment demonstrated 1.4 61.5% HGPRT activity parental line showed least 8% Barr bodies, excluding possibility contamination by Lesch-Nyhan This demonstration comparable that alkylating agent X-irradiation opens study due base-analog substitution