HIV/SIV DNA vaccine combined with protein in a co-immunization protocol elicits highest humoral responses to envelope in mice and macaques.

摘要: Vaccination with HIV/SIV DNAs elicits potent T-cell responses. To improve humoral immune responses, we combined DNA and protein in a co-immunization protocol using vivo electroporation mice macaques. DNA&protein induced higher antibody responses than or alone, prime/protein boost mice. similar levels of cellular as those obtained by only vaccination. The inclusion SIV HIV Env gp120 did not impair the development elicited present vaccine regimen. In macaques, regimen also broader cross-neutralizing activity. Despite improved immunogenicity co-immunization, formulation EM-005 (GLA-SE) adjuvant further increased anti-Env Dissecting contribution EM-005, found that its administration upregulated expression co-stimulatory molecules stimulated cytokine production, especially IL-6, dendritic cells vivo. These terminally differentiated, mature, possibly promote supporting vaccine. Thus, is promising strategy to rapidity development, magnitude potency