Multiplex chemiluminescence microscope imaging of P16INK4A and HPV DNA as biomarker of cervical neoplasia

摘要: Classification of cervical intraepithelial neoplasia (CIN) lesions in low-grade (CIN1) or high-grade (CIN2-3) ones is crucial for optimal patient management, but current histological diagnosis on bioptic samples often hampered by inter-observer variability. To allow objective classification, we have exploited the peculiar characteristics chemiluminescence detection, such as high sensitivity and easy quantification luminescence signal, to perform sequentially same tissue section both an immunohistochemical quantitative detection p16INK4A (a protein marker CIN lesions) situ hybridization human papillomavirus (generally accepted a necessary insufficient cause carcinoma). Different label enzymes (alkaline phosphatase horseradish peroxidase) were employed order avoid any interference between two assays, image analysis was used obtain evaluation sample positivity. The multiplexed method allowed complementary biomarkers provided discrimination different (non-neoplastic, CIN). This assay might thus represent accurate diagnostic test providing important information counseling, selection therapy follow up after surgical treatment.